NTU568 紅麴菌株研究成果 -- The Effect of Monascus Secondary Polyketide Metabolites, Monascin and Ankaflavin, on Adipogenesis and Lipolysis Activity in 3T3-L1
正在加載......
Top
X
 
會員帳號 :
會員密碼 :
忘記密碼  加入會員
 

The Effect of Monascus Secondary Polyketide Metabolites, Monascin and Ankaflavin, on Adipogenesis and Lipolysis Activity in 3T3-L1    HOME > Research Achievement > Publication of monascin and ankaflavin > Improves blood lipids and prevents obesity

The Effect of Monascus Secondary Polyketide Metabolites, Monascin and Ankaflavin, on Adipogenesis and Lipolysis Activity in 3T3-L1

J Agric Food Chem (2010) 58: 12703-12709

The aim of the present work is to investigate the effects of Monascus secondary metabolites, monascin (MS) and ankaflavin (AK), on cell proliferation, adipogenesis, lipolysis and heparin-releasable lipoprotein lipase (HR-LPL) in 3T3-L1 preadipocyte. MS and AK inhibit the proliferation of 3T3-L1 cells in a dose dependent fashion. At 8 μg/mL concentration MS inhibits proliferation for 80.5% after 48 h, whereas the value for AK is 69.2%. Adipogenesis is inhibited by MS and AK without dose-dependency. Triglycerideis decreased 37.1% and 41.1% respectively by treating 0.125 μg/mL MS and AK. Adipocyte-specific transcription factors peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein β (C/EBPβ), C/EBPδ and C/EBP alpha mRNA levels are measured by real-time polymerase chainreaction. The expression of the four transcriptional factors analyzed (PPARγ, C/EBPβ, C/EBPδ and C/EBP alpha) is reduced at the initial and the middle period. At the later period, there is no effect on the expression of PPARγ and C/EBP alpha by treating MS and AK. Further more, both MS and AK increase basal lipolysis of mature adipocytes by 113.2% and 278.3% up regulation, respectively. And both MS and AK reduce the activity of HR-LPL, by 45.3% and 58.1% reduction, respectively. This study reveals for the first time that Monascus secondary metabolites, MS and AK, can prevent the differentiatioin of preadipocyte and stimulate basal lipolysis of mature adipocytes, avoiding the accumulation of lipid.

討論區 友善列印

   Copyright © 2008  All Rights Reserved